| Registro completo |
| Provedor de dados: |
Ciência Rural
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| País: |
Brazil
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| Título: |
Goat ovarian follicles express different levels of mRNA for inhibin-ßA subunit and activin-A stimulates secondary follicle growth in vitro
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| Autores: |
Leitão,Cintia Camurça Fernandes
Costa,José Jackson Nascimento
Saraiva,Márcia Viviane Alves
Araújo,Valdevane Rocha
Figueiredo,José Ricardo
Hurk,Robert van den
Silva,José Roberto Viana
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| Data: |
2013-01-01
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| Ano: |
2013
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| Palavras-chave: |
Pre-antral follicles
Caprine
Culture
Oocyte
Antrum formation
FSH-R
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| Resumo: |
This study investigated the levels of messenger ribonucleic acids (mRNA) for inhibin-ßA subunit in goat primordial, primary and secondary follicles, as well as in cumulus-oocyte complexes (COCs) and mural granulosa / theca cells of antral follicles. The effects of activin-A (100ng mL-1) and/or follicle stimulating hormone (FSH, 50ng mL-1) on growth and expression of mRNA for activin-A and FSH receptor (FSH-R) in secondary follicles cultured for six days were evaluated. The data showed that the expression of inhibin-ßA is lower in secondary follicles than in primary follicles and is higher in large antral follicles than in small antral follicles. After culture, activin-A and/or FSH promoted growth of secondary follicles, while FSH increased the levels of mRNA for inhibin-ßA, and activin-A increased the levels of FSH-R mRNA. In conclusion, mRNA for inhibin-ßA is expressed at different levels in pre-antral and antral follicles and activin-A acts as a stimulator of the FSH-R expression in goat follicles. On its turn, the expression of inhibin-ßA is stimulated by FSH, which together with activin-A promotes secondary follicle growth in-vitro.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000100018
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| Editor: |
Universidade Federal de Santa Maria
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| Relação: |
10.1590/S0103-84782012005000140
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| Formato: |
text/html
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| Fonte: |
Ciência Rural v.43 n.1 2013
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| Direitos: |
info:eu-repo/semantics/openAccess
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